DNA

Part:BBa_K5017010

Designed by: Christos Kalogeropoulos   Group: iGEM23_Patras-Med   (2023-10-11)


Optimized_3_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)

Anti-mesothelin scFv: is a specialized antibody fragment engineered to target the mesothelin protein, which is often overexpressed in pancreatic cancer. The choice of the anti-mesothelin scFv is very critical for the CAR therapy efficiency. Taking into consideration this fact we chose to use a scFv sequence that binds to a juxtamembrane region of mesothelin at the site where it attaches to the cell membrane and is not inactivated by shed MSLN found at high concentrations inside tumors and in ascites and pleural fluid of cancer patients.

This part came from Part:BBa_K5017002 with mutation at CDR-H3 region. ELGGY→ELNGY

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 382
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 382
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 382
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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