Primer

Part:BBa_K5007018

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-06)


pRS426 R-primer

Reverse primer for amplifying pRS426 and pRS425s around the SmaI restriction site, adding homology regions for cloning part BBa_K5007007 through Gibson Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 10
    Illegal PstI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10
    Illegal PstI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 10
    Illegal PstI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 10
    Illegal PstI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
t_m 66°C