Primer

Part:BBa_K5007013

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-06)

ScLEU2 F-primer

Forward primer for amplifying part BBF10K_000471 from FreeGenes’ Open Yeast Collection, adding a 5’ overhang for cloning into the pRS426 plasmid through Gibson Assembly, replacing the URA3 auxotrophy mark and converting it into a similar of pRS425.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
t_m70C