Primer

Part:BBa_K5007011

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-06)

CBD_syn F-primer

Forward primer for amplifying part BBa_K5007008, adding a 5’ overhang for cloning into the pRS425 plasmid through Gibson Assembly. The overhang has homology around the Eco53KI restriction site, inside the LacZɑ gene, allowing for white-blue selection of transformants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
t_m77C