Primer

Part:BBa_K5007010

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-06)

CBD_sub R-primer

Reverse primer for amplifying part BBa_K5007007, adding a 5’ overhang for cloning into the pRS426 plasmid through Gibson Assembly. The overhang has homology around the SmaI restriction site, inside the LacZɑ gene, allowing for white-blue selection of transformants.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
t_m81C