Coding

Part:BBa_K5007005

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-03)


C. sativa Olivetolic Acid Cyclase without stop-codon.

This part codes for Olivetolic Acid Cyclase, responsible for the cyclization of olivetol, generating olivetolic acid, a key precursor in cannabinoid synthesis. This part has the stop-codon removed for fusion into monocystronic composite parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Gagne SJ, Stout JM, Liu E, Boubakir Z, Clark SM, Page JE. Identification of olivetolic acid cyclase from Cannabis sativa reveals a unique catalytic route to plant polyketides. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12811-6. doi: 10.1073/pnas.1200330109. Epub 2012 Jul 16. PMID: 22802619; PMCID: PMC3411943.


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Parameters
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