Coding

Part:BBa_K5007003

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-03)


C. Sativa Olivetol synthase without stop-codon.

This part codes for Cannabis sativa’s Olivetol synthase, responsible for the condensation of Hexanoyl-CoA and 3x malonate molecules to generate olivetol. This part has the stop-codon removed for fusion into monocystronic composite parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1060
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Taura F, Tanaka S, Taguchi C, Fukamizu T, Tanaka H, Shoyama Y, Morimoto S. Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway. FEBS Lett. 2009 Jun 18;583(12):2061-6. doi: 10.1016/j.febslet.2009.05.024. Epub 2009 May 19. PMID: 19454282.


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Parameters
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