Plasmid

Part:BBa_K5005014

Designed by: Yufan Liu   Group: iGEM23_GEC-CHINA   (2023-10-10)


pTarget

It is the target plasmid from the literature, which is the origin plasmid of BBa_K5005002. The target plasmid contains a T7 promoter downstream of the CMV promoter from the pCDH vector, which can help express the target gene. here, we added the EGFP(BBa_K5005001) ORF downstream of the T7 promoter, obtaining pTarget-EGFP; (3) Through site-directed mutagenesis, we converted pTarget-EGFP to pTarget-BFP (BBa_K5005002). More details about primers are on BBa_K5005001 and BBa_K5005002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 294
    Illegal NotI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1987
    Illegal BglII site found at 2053
    Illegal BglII site found at 2094
    Illegal BglII site found at 5518
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 839
    Illegal NgoMIV site found at 1872
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2076
    Illegal BsaI site found at 5500
    Illegal BsaI.rc site found at 792
    Illegal BsaI.rc site found at 3941
    Illegal SapI site found at 2858
    Illegal SapI.rc site found at 6149


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