Coding

Part:BBa_K5005009

Designed by: YunQi Chen   Group: iGEM23_GEC-CHINA   (2023-10-05)


The product of NAMPT point mutation - G384C.

Through patent search, we discovered that some sites on the NAMPT protein have been confirmed through experiments such as prokaryotic expression, protein purification, and enzyme activity assays to enhance the enzymatic activity of NAMPT. Due to the time constraints of the iGEM competition, we were unable to complete the entire process of NAMPT evolution experiments. However, we have successfully constructed these mutated plasmids, which can be used for subsequent enzyme activity validation. As reported by the patent, the Gly to Cys mutation combined with two other mutations mentioned in the patent increases enzyme activity by 35.6%.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 879
    Illegal BglII site found at 1176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1343


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