Plasmid

Part:BBa_K5005005

Designed by: YunQi Chen   Group: iGEM23_GEC-CHINA   (2023-10-05)


Primordial plasmid before point mutation.

NAMPT (BBa_K5005004) is a key rate-limiting enzyme for NAD+ biosynthesis, and it is the gene we aim to evolve in our project. We obtained the gene sequence of NAMPT from published literature and cloned it into pTarget plasmid (BBa_K5005002) that can be expressed in mammalian cells through molecular cloning. This new NAMPT-expressing vector is also compatible with the pEditor system (BBa_K5005003) for directed evolution. This is the plasmid where NAMPT cloned into. More details aboout primers are on the webpage of BBa_K5005004.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5150
    Illegal XbaI site found at 5659
    Illegal SpeI site found at 4780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5156
    Illegal SpeI site found at 4780
    Illegal NotI site found at 6646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3111
    Illegal BglII site found at 6045
    Illegal BglII site found at 6342
    Illegal BglII site found at 8312
    Illegal BglII site found at 8378
    Illegal BglII site found at 8419
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5150
    Illegal XbaI site found at 5659
    Illegal SpeI site found at 4780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5150
    Illegal XbaI site found at 5659
    Illegal SpeI site found at 4780
    Illegal NgoMIV site found at 7164
    Illegal NgoMIV site found at 8197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3093
    Illegal BsaI site found at 8401
    Illegal BsaI.rc site found at 1534
    Illegal BsaI.rc site found at 6509
    Illegal BsaI.rc site found at 7117
    Illegal SapI site found at 451
    Illegal SapI.rc site found at 3742


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