Part:BBa_K4998047

VapB15 Antitoxin composite

Usage and Biology

This part contains the P_QS promoter for the antitoxin (BBa_K4998034), the antitoxin VapB15 gene (BBa_K4998035) and the rrnB T1 terminator (BBa_B0010).

This part contains the sequence of the VapB15 antitoxin gene, which is part of the VapBC15 toxin-antitoxin system, found in the model cyanobacterium Synechocystis sp. PCC 6803. It is produced by the operon vapBC15 of its chromosomal DNA. When produced, the antitoxin VapB15 upregulates the operon’s function by directly binding to the operator, and at the same time destabilizes the toxin, VapC15, because of a toxin-antitoxin complex (TA) formation. When the cell is under stress conditions, the antitoxin levels drop due to the action of proteases and the ability of VapC15 to destabilize the bond between the VapB15 and the promoter region. (This means that less TA complexes are formed and the VapC15 which belong to the VapC-family toxins, acts as a ribonuclease and either prohibits cell growth, or even leads to cell death).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Fei, Q., Gao, E.-B., Liu, B., Wei, Y., & Ning, D. (2018). A toxin-antitoxin system Vapbc15 from synechocystis sp.. PCC 6803 shows distinct regulatory features. Genes, 9(4), 173. https://doi.org/10.3390/genes9040173