Part:BBa_K4998042

TetR under Pgrac promoter

This composite part contains the tetR gene under the Bacillus subtilis derived P_grac promoter (BBa_K1074012), an RBS sequence (BBa_B0034), and the T1 promoter from E.coli rrnB.

TetR proteins are part of bacterial resistance mechanisms to tetracycline (Tc). In the absence of the antibiotic, TetR binds to the operator region of the tetA gene promoter, which encodes a membrane protein that pumps out Tc before it reaches ribosomes. Tc binds to the TetR proteins and releases them from the tetA operator region, allowing the respective gene to be expressed. [1]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Aleksandrov, A., Schuldt, L., Hinrichs, W., & Simonson, T. (2008). Tet repressor induction by tetracycline: A molecular dynamics, continuum electrostatics, and crystallographic study. Journal of Molecular Biology, 378(4), 898–912. https://doi.org/10.1016/j.jmb.2008.03.022