Part:BBa_K4998038

VapC15 gene composite

Usage and Biology

This part consists of the PpsbA1 promoter (BBa_K4998031), an RBS (BBa_K4998024), the gene of the VapC15 toxin (BBa_K4998025), and the rrnB T2 Terminator (BBa_K4998025) terminator. The PpsbA1 promoter is a strong, light-induced, constitutive promoter originating from the Anabaena (Nostoc) PCC 7120 strain [1]. The VapC15 gene expresses a toxin originating from Synechocystis sp. PCC 6803, in our project was used in a kill-switch mechanism in the cyanobacterium Nostoc oryzae TAU-MAC 2710 [2].

Usage and Biology

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 328
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]Chaurasia, A. K., Parasnis, A., & Apte, S. K. (2008). An integrative expression vector for strain improvement and environmental applications of the nitrogen fixing cyanobacterium, anabaena sp. strain PCC7120. Journal of Microbiological Methods, 73(2), 133–141. https://doi.org/10.1016/j.mimet.2008.01.013

[2] Fei, Q., Gao, E.-B., Liu, B., Wei, Y., & Ning, D. (2018). A toxin-antitoxin system Vapbc15 from synechocystis sp. PCC 6803 shows distinct regulatory features. Genes, 9(4), 173. https://doi.org/10.3390/genes9040173