Part:BBa_K4998037

LuxR gene composite

Usage and Biology

This part consists of a trc promoter (BBa_K4998021), an RBS (BBa_K103015), the LuxR gene (BBa_K4998022), and the ECK120034435 terminator (BBa_K4998023). The trc promoter is a strong, IPTG-induced hybrid of the lac and trp promoters [1]. In our design, it is responsible for the expression of the LuxR a gene that results in a protein that is expressed constantly and has the ability to bind to AHL (N-Acyl homoserine lactone) thus taking part in quorum sensing systems [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 441
  

References

[1] Kokarakis, E. J., Rillema, R., Ducat, D. C., & Sakkos, J. K. (2022). Developing cyanobacterial quorum sensing toolkits: Toward interspecies coordination in mixed autotroph/heterotroph communities. ACS Synthetic Biology, 12(1), 265–276. https://doi.org/10.1021/acssynbio.2c00527