Terminator

Part:BBa_K4998033

Designed by: Asimenia Ioannidou   Group: iGEM23_thessaloniki   (2023-10-09)


trp terminator

Usage and Biology

This part contains an E. coli tryptophan operon terminator sequence. In our project, it was used as a terminator after the hetr gene that was expressed in Nostoc oryzae TAU-MAC 2710.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

References

[1] Wu, A. M., & Platt, T. (1978). Transcription termination: Nucleotide sequence at 3′ end of tryptophan operon in Escherichia coli. Proceedings of the National Academy of Sciences, 75(11), 5442–5446. https://doi.org/10.1073/pnas.75.11.5442

[edit]
Categories
Parameters
None