Regulatory

Part:BBa_K4998031

Designed by: Rafail Andreou   Group: iGEM23_thessaloniki   (2023-10-09)

psbA1 promoter

Usage and Biology

Cyanobacterial promoter for constitutive gene expression in cyanobacterial strains. A light-inducible, strong promoter, derived from the Anabaena (Nostoc) sp. PCC 7120 strain. [1] Used for hetR (BBa….) gene overexpression, in the Nostoc oryzae TAU-MAC 2710 strain (iGEM Thessaloniki 2023 project, “Euphoresis”).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

References

[1] Chaurasia, A. K., Parasnis, A., & Apte, S. K. (2008). An integrative expression vector for strain improvement and environmental applications of the nitrogen fixing cyanobacterium, Anabaena sp. strain PCC7120. Journal of Microbiological Methods, 73(2), 133–141. https://doi.org/10.1016/j.mimet.2008.01.013

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Categories
Parameters
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