Part:BBa_K4998029

bsrG toxin gene

Usage and Biology

This part includes the mRNA sequence of the type 1 Bacillus subtilis toxin, bsrG. bsrG is a 38-amino acid, toxic and hydrophobic peptide that leads to B. subtilis’ cell lysis. Naturally, it participates along with the SR4 RNA antitoxin in the Bacillus subtilis’ temperature-dependent toxin-antitoxin system. [1]

The bsrG mRNA sequence consists of 227 bases. An RBS starts from the base 50 and is a 4bp, double-stranded region (GGGG). The translation unit starts from the base 61 and it translates to the 38-amino acid toxin peptide. [1]

The bsrG toxin mRNA is utilized in iGEM Thessaloniki’s 2023 project, “Euphoresis”, as part for the Bacillus subtilis WB800N strain kill switch mechanism.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Jahn, N., & Brantl, S. (2013). One antitoxin—two functions: SR4 controls toxin mrna decay and translation. Nucleic Acids Research, 41(21), 9870–9880. https://doi.org/10.1093/nar/gkt735