Primer

Part:BBa_K4998002

Designed by: Markos Mathioudakis   Group: iGEM23_thessaloniki   (2023-10-06)

Laccase gene left primer

This is the left primer of the laccase gene. We used this primer for PCR amplification of the laccase gene, which contains the coding region for laccase enzyme. More information about the laccase part can be found in


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 9
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


Characteristics

Tm: 59.33 GC%: 55.00

References

https://primer3.ut.ee/

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Parameters
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