Primer

Part:BBa_K4998000

Designed by: Markos Mathioudakis   Group: iGEM23_thessaloniki   (2023-10-06)

LuxI gene left primer

This is the left primer of the LuxI gene. We used this primer for PCR amplification of the LuxI gene, which contains the coding region for the AHL synthase enzyme. More information about the LuxI part can be found in

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characteristics

Tm: 59.01 GC%: 55.00

References

https://primer3.ut.ee/

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Categories
Parameters
None