Plasmid

Part:BBa_K4974018

Designed by: Benjamin Li   Group: iGEM23_NYCEmpireState   (2023-10-07)


pET-28a(+)-FGF2-G3 with alpha amylase

This part consists of the commercially available pET-28a(+)-FGF2-G3fi plasmid from Addgene (cat. # 135521) and inserted the alpha amylase sequence by removing the MCS. pET-28a(+)-FGF2-G3fi has multiple cloning sites containing XbaI and XhoI restriction sites. In between those cloning sites, teams can clone the codon-optimized human alpha-amylase sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 656
    Illegal XbaI site found at 47
    Illegal PstI site found at 373
    Illegal PstI site found at 7194
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 656
    Illegal NheI site found at 151
    Illegal PstI site found at 373
    Illegal PstI site found at 7194
    Illegal NotI site found at 681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 656
    Illegal BglII site found at 5812
    Illegal BamHI site found at 184
    Illegal XhoI site found at 690
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 656
    Illegal XbaI site found at 47
    Illegal PstI site found at 373
    Illegal PstI site found at 7194
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 656
    Illegal XbaI site found at 47
    Illegal PstI site found at 373
    Illegal PstI site found at 7194
    Illegal NgoMIV site found at 989
    Illegal NgoMIV site found at 4032
    Illegal NgoMIV site found at 4192
    Illegal NgoMIV site found at 5780
    Illegal NgoMIV site found at 6201
    Illegal NgoMIV site found at 7208
    Illegal AgeI site found at 604
    Illegal AgeI site found at 6303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3114


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