Part:BBa_K4974004

Human Alpha-Amylase

This part is the coding sequence for the alpha-amylase protein. Alpha-amylase is involved in the process of starch digestion. Alpha-amylase hydrolyzes the α-1,4-glycosidic bonds in starch and related polysaccharides such as amylose, amylopectin, and glycogen. The protein is primarily produced by the pancreas and salivary glands in mammals like humans. Alpha-amylase has also been shown to play a role in the human body's acute stress response. The HPA axis mediates the production of alpha-amylase, and causes a significant increase in salivary alpha-amylase production in response to acute stress.

This part can be expressed in Gibson cloning vectors to produce alpha-amylase protein in BL-21 e. coli, as was done by the 2023 NYCEmpireState team.

If a team orders the human alpha amylase sequence, they can dissolve it in the proper solvent (i.e. ddH2O or TE). To increase the efficiency and the amount of the product, conduct a PCR assay using following primers: 5’ CTATAGGGGAATTGTGAGCGG 3’ (forward) and 5’ TAGCAGCCGGATCTCAGTG 3’ (reverse)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1363
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1363
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1363
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1363
  Illegal NgoMIV site found at 370
  Illegal NgoMIV site found at 1377
  Illegal AgeI site found at 472
  
• 1000
  COMPATIBLE WITH RFC[1000]