Primer

Part:BBa_K497019

Designed by: Joanna Hare   Group: iGEM10_Macquarie_Australia   (2010-10-27)

Reverse primer Deinococcus radiodurans bacteriophytochrome gene for insertion after heme oxygenase

This is the reverse primer for full amplification of bacteriophytochrome gene of D. radiodurans. This has an additional sequence added so that the bacteriophytochrome gene can be inserted in an operon after a heme oxygeanase gene. Based on sequence from National Center for Biotechnology Information.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 11
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None