Plasmid_Backbone

Part:BBa_K4968012

Designed by: Shouye Zhu   Group: iGEM23_XJTLU-CHINA   (2023-10-09)

pUC_GW_KanR

pUC-GW-Kan is a common used plasmid cloning vector in E. coli. The vector length is 2,626 bp. And GW represents "Gateway" technology, which is commonly used for efficient gene cloning and expression. Gateway technology allows researchers to easily move gene fragments into different plasmids, achieving targeted cloning and expression of target genes.The plasmid contains the kanamycin antibiotic resistance gene for screening cells carrying this plasmid when cultivating Escherichia coli.

Figure 1. The plasmid map of pUC-GW-KanR

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 25
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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