Part:BBa_K4968005

Optimized AG4

AG4 is a synthetic 12-peptide that can specifically bind silver ions. We take advantage of the relatively small mass of the AG4 protein and its ability to adsorb silver ions. It is combined with the curly fiber backbone formed by the CsgA protein to form a CsgA-AG4 fusion protein curli fiber membrane.

AG4 serves as a protein with potential applications in adsorbing valuable silver ions from the environment (Seker and Demir, 2011).

Codon Optimization

We optimized the codons of AG4 to improve the expression efficiency in the target strain. Below is the DNA sequence change of AG4 by optimizing codons.

AG4 sequence before Codon-optimization:

    1  AACCCGAGCA 
   11  GCCTGTTTCG 
   21  CTATCTGCCG 
   31  AGCGATTAA
  

AG4 sequence after Codon-optimization:

    1  AATCCGAGTA
   11  GTTTATTTCG
   21  CTATCTCCCG
   31  AGCGACTAA
 

Figure 1 | AG4 peptides (yellow) with the linker (green).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Source

The sequence of AG4 is from the literature “Solution structure of peptide AG4 used to form silver nanoparticles” (Asn1-Pro-Ser-Ser-Leu-Phe-Arg-Tyr-Leu-Pro-Ser-Asp)

References

Seker, U.O.S. and Demir, H.V. (2011) ‘Material Binding Peptides for Nanotechnolgy’ Molecules 16(2),1426-1451. Available at: https://doi.org/10.3390/molecules16021426