Part:BBa_K4960040
pPVC promoter->pvc1-pvc12->pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD->pvc14-pvc16
A system for targeted delivery of target proteins.
Profile
Name: pvc1-pvc12、pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD、pvc14-pvc16
Origin: Synthetic
Properties: A system for targeted delivery of target proteins.
Usage and Biology
Mutant Pvc13-E01-DARPin fused with the DARPin E01 structural domain can enhance the binding ability of PVCs to cells, ultimately enabling targeted delivery of fat proteins. Pvc13-E01-DARPin mutant-based constructs of PVCs particles can specifically target cells with EGFR receptors[1]. In subsequent cell experiments, we used Hela and HEK-293T with high EGFR expression to ensure the targeting of PVC.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7406
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2407
Illegal BglII site found at 11264
Illegal BglII site found at 14099
Illegal BglII site found at 15464
Illegal BglII site found at 17422
Illegal XhoI site found at 2691 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 11792
Illegal NgoMIV site found at 17953
Illegal NgoMIV site found at 18092
Illegal AgeI site found at 6704
Illegal AgeI site found at 9641
Illegal AgeI site found at 10327
Illegal AgeI site found at 10468
Illegal AgeI site found at 11576
Illegal AgeI site found at 18391
Illegal AgeI site found at 20079 - 1000COMPATIBLE WITH RFC[1000]
Tube:pvc1, pvc5, pvc7
Sheath:pvc2, pvc3, pvc4
Sheath terminator:pvc15
Tail fibres:pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD
Baseplate:pvc9, pvc11, pvc12
Spike:pvc8, pvc10
Assembly method:3A Assembly
References
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364.
| None |