Coding

Part:BBa_K4960007

Designed by: Ruoxi Lv   Group: iGEM23_NUDT-CHINA   (2023-10-10)


pvc7
guide the synthesis of the PVC tube initiator

Profile

Name: pvc7
Base Pairs: 690 bp
Origin: Photorhabdus
Properties: guide the synthesis of the PVC tube initiator


Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc7 produces pvc7 hexameric rings to form the first two initiator rings in the baseplate end of the PVC tube with pvc5 through opposite surface charges and electrostatic attraction.In the meantime,the pvc7 layer (appear to be fusion protein) docks onto the b-barrel ring (pseudo-6-fold) of Pvc8 to help form a continuous central spike extending from the inner tube.
Furthermore, pvc7 is encircled by pvc9 and acts as a sheath initiator collectively to prepare for the contraction in the delivery process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.

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Categories
Parameters
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