Part:BBa_K4953001
N56 aptamer
This part is a single-stranded DNA aptamer reported to have a binding specificity to CEA protein, according to the study by Dasari et al.[1]. This team has discovered this aptamer through the Systematic Evolution of Ligands by EXponential Enrichment (SELEX) experiment.
We further validated the binding of N56 to the CEA protein using experimental techniques such as EMSA and PAD. In particular, through PAD, we discovered that N56 binds to CEA perpendicularly, indicating its suitability for diagnostic systems like LFA.
N56 aptamer exhibits high specificity for CEA [1] and, being in aptamer form, has the advantage of greater accessibility compared to antibodies. Additionally, we have characterized the binding pattern of the N56-CEA complex. Therefore, future iGEM teams aiming to target this protein or utilize it as a biomarker may have a simpler and more convenient method at their disposal.
[Reference]
[1] E. W. Orava, Aws Abdul-Wahid, E. J. Huang, Amirul Islam Mallick, and J. Gariépy, “Blocking the attachment of cancer cells in vivo with DNA aptamers displaying anti-adhesive properties against the carcinoembryonic antigen,” Molecular Oncology, vol. 7, no. 4, pp. 799–811, Aug. 2013, doi: https://doi.org/10.1016/j.molonc.2013.03.005.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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