Composite
Part:BBa_K4945005
Designed by: Na Wang Group: iGEM23_BJYUAN-CHINA (2023-10-06)
butyrate->red fluorescence
We combined promoter pPchA (BBa_4945001), PchA(BBa_4945003), promoter LEE(BBa_4945004) with the reporter gene mRFP (BBA_E1010) to create an butyrate sensitive in vivo biosensor. The E. coli leucine-responsive regulatory protein (Lrp) forms a binary complex with butyrate, which can bind to the promoter region of pchA (BBa_4945001) and then initiate the transcriptional expression of pchA(BBa_4945003).The PchA regulator is able to bind to the promoter of BBa_4945004, activating transcription of the mRFP gene under control of BBa_4945004. mRFP is a red fluorescent protein from the Discosoma striata (coral) which has an excitation maximum hat 584 nm and an emission maximum 607 nm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1695
Illegal AgeI site found at 1807 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 729
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Categories
Parameters
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