Composite

Part:BBa_K4945005

Designed by: Na Wang   Group: iGEM23_BJYUAN-CHINA   (2023-10-06)


butyrate->red fluorescence

We combined promoter pPchA (BBa_4945001), PchA(BBa_4945003), promoter LEE(BBa_4945004) with the reporter gene mRFP (BBA_E1010) to create an butyrate sensitive in vivo biosensor. The E. coli leucine-responsive regulatory protein (Lrp) forms a binary complex with butyrate, which can bind to the promoter region of pchA (BBa_4945001) and then initiate the transcriptional expression of pchA(BBa_4945003).The PchA regulator is able to bind to the promoter of BBa_4945004, activating transcription of the mRFP gene under control of BBa_4945004. mRFP is a red fluorescent protein from the Discosoma striata (coral) which has an excitation maximum hat 584 nm and an emission maximum 607 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1695
    Illegal AgeI site found at 1807
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 729


[edit]
Categories
Parameters
None