Regulatory

Part:BBa_K4942002

Designed by: Huang Xinqi   Group: iGEM23_SHSID-China   (2023-08-10)


luxI


Documentation for BBa_K4942002

BBa_K4942002 (luxI)

Profile

  • Name: luxI
  • Base Pairs: 582bp
  • Origin: Aliivibrio logei, synthetic
  • Properties: LuxI (LuxI-Family Autoinducer Synthase) is an enzyme that belongs to the LuxI family of autoinductive synthases. LuxI family enzymes are widely distributed in bacteria, especially Gram-negative bacteria.

Usage and Biology

LuxI enzyme plays a role in the synthesis of bacterial self-sensing signal molecules. These signaling molecules are usually N-acyl-Homoserine Lactone (AHL). Bacteria achieve quorum sensing and information sharing among bacterial populations by producing and detecting these signal molecules AHL. It is particularly important in biofilm formation and bacterial populations that cause biomass attachment. When the number of bacteria in the bacterial population reaches a certain threshold, LuxI catalyzes the conversion of substrates into AHL signaling molecules and leads to other bacterial sensing signals, thereby controlling the expression of specific genes[1].

Reference

  1. N M K, A S K, Ancha B, et al. A combination of luxR1 and luxR2 genes activates Pr-promoters of psychrophilic Aliivibrio logei lux-operon independently of chaperonin GroEL/ES and protease Lon at high concentrations of autoinducer. Biochemical and biophysical research communications, 2016, 473(4).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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