Plasmid

Part:BBa_K4941040

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pet28a- T7 promoter-His tag- T7 terminator- f1 origin- ColE1 pBR322 origin- kan-lac I

Empty plasmid vector for gene expression. The plasmid carries a kana screening marker for kana resistance and a T7 promoter to initiate gene expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 232
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None