Plasmid

Part:BBa_K4941039

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pylxp-CEN1-1-ORI1001-Rep Origin (pMB1)-AmpR-Leu2 marker

Empty plasmid vector for gene expression. The plasmid carries an AmpR screening marker and a leu screening marker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3074
    Illegal XhoI site found at 3103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3960
    Illegal AgeI site found at 2489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1668
    Illegal BsaI site found at 3245
    Illegal BsaI.rc site found at 3160
    Illegal BsaI.rc site found at 3443
    Illegal SapI.rc site found at 2691


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