Plasmid
Part:BBa_K4941037
Designed by: Xinyu Chen Group: iGEM23_ZJFH-Nanjing (2023-10-08)
pylxp-CEN1-1-pTEF-Incomplete Intron-ylER-XPR2_terminator -ORI1001-Rep Origin (pMB1)-AmpR-Leu2 marker
Plasmid for ylER expression.The plasmid bears an AmpR screening marker and the endogenous promoter pTEF initiates ylER expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 976
Illegal XhoI site found at 1374
Illegal XhoI site found at 5013
Illegal XhoI site found at 5042 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5899
Illegal AgeI site found at 1853
Illegal AgeI site found at 4428 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 822
Illegal BsaI site found at 3607
Illegal BsaI site found at 5184
Illegal BsaI.rc site found at 237
Illegal BsaI.rc site found at 5099
Illegal BsaI.rc site found at 5382
Illegal SapI site found at 1124
Illegal SapI.rc site found at 4630
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Categories
Parameters
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