Plasmid

Part:BBa_K4941035

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pet28a- T7 promoter- DnXR- His tag- T7 terminator- f1 origin- ColE1 pBR322 origin- kan-lac I

The pet28a plasmid for DnXR expression with a kanamycin resistance gene can be used for colony screening. lac I expresses a inhibitor and thus inhibits target gene expression, which can be lifted by the addition of IPTG to induce DnXR gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 576
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 576
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 576
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 576
    Illegal NgoMIV site found at 1201
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None