Plasmid

Part:BBa_K4941030

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pylxp-CEN1-1-pTEF-Incomplete Intron-URA-XPR2_terminator -ORI1001-Rep Origin (pMB1)-AmpR-Leu2 marker

Plasmid for URA expression with AmpR screening marker. The endogenous promoter pTEF initiates URA expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 784
    Illegal XhoI site found at 817
    Illegal XhoI site found at 4902
    Illegal XhoI site found at 4931
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 972
    Illegal NgoMIV site found at 5788
    Illegal AgeI site found at 1742
    Illegal AgeI site found at 4317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3496
    Illegal BsaI site found at 5073
    Illegal BsaI.rc site found at 237
    Illegal BsaI.rc site found at 4988
    Illegal BsaI.rc site found at 5271
    Illegal SapI.rc site found at 4519


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Categories
Parameters
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