Coding

Part:BBa_K4940015

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-11)


GlcDH-II

GlcDH-II is a coding sequence of glucose dehydrogenase2 from Bacillus megaterium. This enzyme can convert the unphosphorylated glucose in the cytoplasm to gluconolactone, which can further hydrolyzes to gluconate spontaneously. During the whole process, NADPH can also be generated (1). Combined with GLF and P450BM-3 QM, a system can be constructed to effectively and continuously degrade α-pinene to α-pinene oxide.

Usage and Biology

More specific information can be found in BBa K4940002. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 371
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None