Composite

Part:BBa_K4940012

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-11)


Transcription unit of GLF-his This is a transcription unit in pQE-80L-Kan, of gene GLF-his. Transcription will be initiated through T5 promoter, and terminated by Lambda t0 terminator. Additionally, lac operator can control gene expression only possible when induced by IPTG, which promises biological safety.

Usage and Biology

More specific information can be found in BBa_K4940003. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 506
    Illegal NgoMIV site found at 590
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 989
    Illegal NgoMIV site found at 1025
    Illegal AgeI site found at 545
    Illegal AgeI site found at 701
    Illegal AgeI site found at 713
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None