![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4940010
Designed by: Kuang Minjie Group: iGEM23_iZJU-China (2023-10-11)
Transcription unit of Prα-POL-his
This is a transcription unit in pET-28a, of gene Prα-POL-his tag. Transcription will be initiated through T7 promoter, and terminated by T7 terminator. Additionally, lac operator can control gene expression only possible when induced by IPTG, which promises biological safety.
Usage and Biology
More specific information can be found in BBa_K4940003.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 140
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 346
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |