Part:BBa_K4939002
UAS-pBmo
A butanol sensitive sigma-54 dependant promoter from Thauera butanivorans, with an included upstream activation site 193 bp upstream of the -24 and -12 RNAP binding sites.
Originally isolated from sludge at a Japanese oil refinery, Thauera butanivorans is a gram negative alkanotroph that can metabolise C2-C9 alkanes. The metabolic operon with which the cells can carry out this metabolism is under the control of pBmo - a butanol sensitive, σ54 dependent promoter that itself is regulated by the BmoR repressor (an alcohol-responsive bacterial enhancer protein (bEBP)). In typical σ54 fashion, the activation of the associated operon involves an upstream activation sequence (UAS) some 193 bp upstream of the RNAP binding site. Therefore, BmoR binding triggers significant DNA bending to achieve gene activation. Thus, for the use of a functional butanol biosensor in vivo, the strain in question must also contain the UAS and the transcriptional unit for BmoR. Butanol biosensing has been achieved in E. coli using this system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 298
- 1000COMPATIBLE WITH RFC[1000]
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