Composite

Part:BBa_K4937026

Designed by: Chen Wang   Group: iGEM23_SCU-BSE-China   (2023-09-27)


TDH3p-SPE3-PRM9t

This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.

24-1.png

We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of S. cerevisiae and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2).

24-2.png 24-3.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1609
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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