Regulatory
Part:BBa_K4907031
Designed by: Xinyi Yang Group: iGEM23_XMU-China (2023-09-13)
I0500weak
Usage and Biology
pBAD/araC promoter consists of two structures, namely araC and araBAD promoter. We found that BBa_K206001 and araBAD promoter partially overlapped, so we tried to combine BBa_K206001, the non-overlapping part, and araC to build an I0500-weak promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1204
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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Categories
Parameters
None |