Signalling

Part:BBa_K4905019

Designed by: Jay Beeren   Group: iGEM23_TU-Eindhoven   (2023-09-20)


The Ompf sequence is a signaling peptide allowing for translocation of a protein to the periplasm

The E.coli K-12 strain possesses a protein, called the outer membrane protein F (Ompf). The precursor of this protein contains a signaling peptide 22 amino acids in length, which is the Ompf signaling sequence[1]. This sequence is used for translocating proteins from the cytoplasm to the periplasm[2]. For this reason, the Ompf sequence can be fused to other proteins for the secretion into the periplasm[3].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Inokuchi K, Mutoh N, Matsuyama S, Mizushima S (1982) Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res 10:6957-6968

[2] Vlasuk GP, Inouye S, Ito H, Itakura K, Inouye M (1983) Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli. J Biol Chem 258:7141-7148

[3] Yamamoto, T, Okawa N.,Endo T., and Kaji A. (1991) Expression of chimeric ras protein with OmpF signal peptide in Escherichia coli: localization of OmpF fusion protein in the inner membrane. Appl Microbiol BiotechnoZ 35:615-621



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