Part:BBa_K4904000

2X UAS promoter

Basic Part: BBa_K4904000 (2X UAS promoter)

Profile

Name: 2X UAS promoter

Base Pairs: 114bp

Origin: Plants, Synthetic

Properties

GAL4/UAS is a gene expression regulatory system present in yeast.

Usage and Biology

GAL4/UAS is a gene expression regulatory system present in yeast. UAS is the abbreviation of upstream activating sequence. GAL4 is a transcriptional regulator whose Binding domain (BD) binds to the UAS sequence, and its Activity domain (AD) binds to the promoter region, thus inducing gene expression. The GAL4/UAS system has been widely used in various gene regulation studies [1].

Figure 1. The 2X UAS promoter

References:

1. Ge W D. Preliminary establishment of a technique for analyzing human gene function in the Drosophila GAL4-UAS system [D]. Fudan University, Shanghai. 2000. DOI:10.7666/d.y362704.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 66
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 66
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 66
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 66
  
• 1000
  COMPATIBLE WITH RFC[1000]