RNA
Part:BBa_K4899002
Designed by: Morten Pedersen Hauger Group: iGEM23_UiOslo-Norway (2023-10-04)
miniaturized CAS12f guide RNA
a miniaturized sgRNA for Cas12f that targets a DNA sequence containing the sequence of the micro RNA miR199b within the miR199b sequence.
Usage and Biology
A sgRNA for cas12f and dcasMINI that has been subjected to hairpin truncation to reduce its size, it was created by the fusion of the tracrRNA and crRNA used in the generation of cas12fs natural guide RNA (Xu et.al). It serves to target the site of cleavage and strongest binding for cas12f and dCasMINI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
characterization
[edit]
Categories
Parameters
None |