Composite

Part:BBa_K4898001

Designed by: Aayushi Agrawal   Group: iGEM23_IIT-Delhi   (2023-10-12)


Alasan biosurfactant producing gene AlnA under strong inducible promoter Ptac

Background

Ptac is induced using IPTG to produce alasan. The speciality of this part is that even a cloning strain like DH5-alpha is able to express the compound with high yields due the high strength of the promoter. The strain was able to produce a more concentrated solution of the biosurfactant with merely overnight incubation in 37 degree Celsius than the wild type strain after 7 day incubation.

Part Uses

1. Production of alasan on a industrial scale. 2. Can be used in a shuttle vector as Ptac promoter works in most of the reported organisms

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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