Part:BBa_K4897021

Caf1-His-556 antibacterial peptide

What is it?

Caf1-His-556 Antibacterial peptide is a protein that combines the his-tag and AMP functions. Capsular antigen fragment 1 (Caf1) is a thermally reformable protein that forms polymers under cooled conditions while staying in a monomeric form when heated. The his-tag component functions in joining the polymers together to form a net as his-tag antibodies subsequently bind individual his-tags together. Protein A/G also binds to the his-tag antibodies, subsequently attracting protein A/G magnetic beads that can be attracted by magnets. This property later facilitates the removal of the caf1 network. 556 antibacterial peptide is an antibacterial peptide that effectively inhibits P. acne.

Usage and Biology

Together, this part can be used to inhibit P. acne effectively by inserting it into facial pores containing the bacteria. By first trapping P. acne in a complex net through the his-tags and corresponding antibodies, 556 antibacterial peptide can eliminate P. acne. Finally, protein A/G and the protein A/G magnetic beads attached to the caf1 work to remove the P. acne remains and the proteins from facial pores.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Dura, Gema, et al. “A thermally reformable protein polymer.” Chem, vol. 6, no. 11, 5 Nov. 2020, pp. 3132–3151, https://doi.org/10.1016/j.chempr.2020.09.020. [2] “His-Tagged Proteins–Production and Purification: Thermo Fisher Scientific.” ThermoFisher, www.thermofisher.cn/cn/zh/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/his-tagged-proteins-production-purification.html. Accessed 9 Oct. 2023. [3] Zhang, Q.-Y., Yan, Z.-B., Meng, Y.-M., Hong, X.-Y., Shao, G., Ma, J.-J., … Fu, C.-Y. (2021). Antimicrobial peptides: mechanism of action, activity and clinical potential. Military Medical Research, 8, 48. https://doi.org/10.1186/s40779-021-00343-2.