DNA

Part:BBa_K4897015

Designed by: ZHANG TONG   Group: iGEM23_BS-United-China   (2023-10-07)


sgRNA-A2

With reference to part BBa_K4329007, another sgRNA-A2 (sequence AAAGTCGCAATTGTATGCAC) is designed by our team BS_United_China in 2022 to target the ATP synthase subunit C also used, so that when it is combined with CAS9, two parts of the ATP synthase is destroyed, guaranteeing the death of the powerhouse of the bacteria.

Usage and Biology

EL222 in our blue light suicide system will activate the expression of CAS9 and its sgRNA sequence (here sgRNA-A2). The gene editing process takes place after that, disrupting two of the most crucial parts of the bacterial cell’s metabolic pathways, energy and protein production, which eventually cause the death of bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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