Coding
Part:BBa_K4883001
Designed by: Yuqi Fu Group: iGEM23_Hangzhou-BioX (2023-06-29)
RIB1, GTP cyclohydrolase II from Saccharomyces cerevisiae
RIB1 encodes for GTP cyclohydrolase II in S. cerevisiae. This enzyme catalyzes the first step of the riboflavin biosynthesis pathway, transforming GTP to 2,5-diamino-6-(5-phospho-D-ribosylamino)-pyrimidin-4(3H)-one (DARPP) (Gudipati et al., 2014).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 428
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 448
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
RIB1 is a riboflavin synthase. Overexpression of RIB1 could lead to the overproduction of vitamin B2 in S. cerevisiae.
References
Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015.
[edit]
Categories
Parameters
None |