Part:BBa_K4879002

CvFAP

The fatty acid photodecarboxylase from Chlorella variabilis. [1]

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Usage and Biology

The fatty acid photodecarboxylase, found mainly in the chloroplasts of microalga Chlorella variabilis, catalyses the decarboxylation of free fatty acids into n-alkanes or n-alkenes in the presence of blue light. The reaction is initiated by electron abstraction from the fatty acid to a photo-excited cofactor flavin adenine dinucleotide (FAD).

The direct conversion of free fatty acids (FFAs) into n-alkanes and n-alkenes is an essential feature of our project as it bypasses a series of more expensive, intensive and CO₂ emitting chemical hydroprocessing steps in fuel (hydrocarbon) production.

The protein structure of CvFAP, as predicted by AlphaFold

Design

The transcriptional unit for CvFAP (BBa_K4879008) is designed with the coding sequence flanked by the TEF1 promoter (BBa_K3629001) upstream and the XPR2 terminator (BBa_K3629004) downstream.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 259
  Illegal PstI site found at 691
  Illegal PstI site found at 958
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 259
  Illegal PstI site found at 691
  Illegal PstI site found at 958
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1424
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 259
  Illegal PstI site found at 691
  Illegal PstI site found at 958
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 259
  Illegal PstI site found at 691
  Illegal PstI site found at 958
  Illegal NgoMIV site found at 1579
  Illegal AgeI site found at 1555
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Bruder, S., Moldenhauer, E.J., Lemke, R.D. et al. Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica. Biotechnol Biofuels 12, 202 (2019). https://doi.org/10.1186/s13068-019-1542-4