Plasmid_Backbone

Part:BBa_K4876014

Designed by: Jin Luming   Group: iGEM23_Zhejiang-United   (2023-09-28)


gRNA

gRNA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 307
    Illegal XbaI site found at 313
    Illegal SpeI site found at 218
    Illegal PstI site found at 325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 307
    Illegal NheI site found at 195
    Illegal SpeI site found at 218
    Illegal PstI site found at 325
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 307
    Illegal BglII site found at 337
    Illegal BamHI site found at 183
    Illegal XhoI site found at 361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 307
    Illegal XbaI site found at 313
    Illegal SpeI site found at 218
    Illegal PstI site found at 325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 307
    Illegal XbaI site found at 313
    Illegal SpeI site found at 218
    Illegal PstI site found at 325
    Illegal NgoMIV site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None