Plasmid

Part:BBa_K4876007

Designed by: Jin Luming   Group: iGEM23_Zhejiang-United   (2023-09-28)


gRNA-gnTt

gRNA-gnTt

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 327
    Illegal XbaI site found at 333
    Illegal SpeI site found at 218
    Illegal PstI site found at 345
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 327
    Illegal NheI site found at 195
    Illegal SpeI site found at 218
    Illegal PstI site found at 345
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 327
    Illegal BglII site found at 357
    Illegal BamHI site found at 183
    Illegal XhoI site found at 381
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 327
    Illegal XbaI site found at 333
    Illegal SpeI site found at 218
    Illegal PstI site found at 345
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 327
    Illegal XbaI site found at 333
    Illegal SpeI site found at 218
    Illegal PstI site found at 345
    Illegal NgoMIV site found at 1152
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None